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KMID : 1189420200330020100
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2020 Volume.33 No. 2 p.100 ~ p.111
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Anti-inflammatory mechanism and Anti-oxidant Effects of Naesohwangryun-tang in LPS-Stimulated RAW 264.7 Macrophage Cells
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Jeon Seon-Hong
Kim Tae-Jun
Kim Yong-Min
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Abstract
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Objectives : The aim of experiment is to examine anti-inflammatory effect and anti-oxidant effect of Naesohwangryun-tang (NSHRT) in LPS-stimulated RAW264.7 macrophage cells.
Methods : In the present study, The cell viability was performed by MTT assay. Nitric oxide (NO) production and prostaglandin E2 (PGE2) synthesis were performed by NO assay and ELISA KIT. The anti-oxidant effect was performed by DPPH and ABTS radical scavenging activity. The inhibitory effects of pro-inflammatory mediators and cytokines were confirmed by realtime PCR and western blotting.
Results : NSHRT was no cytotoxicity at treated group. NO and PGE2 production were inhibited compared to the LPS treated group and also mRNA and protein expressions were significantly decreased compared to the LPS treated group.
Conclusions : According to the above experiments, we confirmed that NSHRT has anti-inflammatory and anti-oxidant effects. It is suggested that NSHRT is potential ingredient of skin diseases.
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KEYWORD
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Naesohwangryun-tang (NSHRT), Anti-inflammatory. Anti-oxidant, NO, COX-2
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